Reproducing the original dandelion method/paper1 years ago
Load the required libraries | Load data | Filter the data | Milo object and neighbourhood graph construction | Construct UMAP on milo neighbor graph | Construct pseudobulked VDJ feature space | TCR trajectory inference using Absorbing Markov Chain | Define root and branch tips | Construct diffusion map | Compute diffussion pseudotime on diffusion map | Markov chain construction on the pseudobulk VDJ feature space | Visualising branch probabilities | Transfer | Project pseudobulk data to each cell | Visualise the trajectory data on a per cell basis | Session info